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PhytoCeutica,
Inc. 5 Science Park, Suite 13 New Haven,
CT 06511
Phone: 203-777-3462 Fax: 203-777-3538 E-mail: info@phytoceutica.com |
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Figure 1: LC/MS
characterization of PHY906. Utilizing high resolution MS
(green), a far more detailed spectrum can be obtained of the phytochemicals
present in PHY906 than with conventional UV/VIS detection (red).
This information rich methodology allows the identification
of more than 90 of the observed 125 phytochemical peaks. Note how
single peaks in the UV/VIS trace (red) can be resolved into multiple
phytochemical peaks in the high-resolution mass dimension |
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> Chemical Fingerprinting >
Genomic Bioreponse Profiling _____________________________________________ |
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Chemical Fingerprinting
In addition to the standard liquid chromatography
with UV-VIS or MS detection for quantitative detection of single marker
standards, PhytoCeutica has developed a rapid method to identify and
characterize a broad range of low molecular weight phytochemicals
in the range of 100-1000 amu. This method combines high sensitivity
capillary (LC) coupled with high resolution mass spectrometry to provide
a high resolution molecular fingerprint that, when coupled to a comprehensive
database of phytochemicals, can be used both for accurate phytochemical
identification and for the creation of detailed phytochemical fingerprints
for QC comparisons. These fingerprints
can form the foundation as a signature profile for standardizing the
botanical extract that defines the clinical batch for later batch-to-batch
comparisons, as a reference to study stability, and as a comprehensive
dataset to identify potential adulterated drugs or chemical contaminants.
An illustration of the approach is described in Figure 1, using
our drug candidate PHY906.
PhytoCeutica’s software package, PhytoViewerCA™,
has been written to analyze chemical fingerprint patterns, identify
the compounds, define the signature pattern, calculate similarity
coefficients, and assign quantitative pattern cut-offs acceptance
or rejection of manufactured batches. |
